Identification and quantification of bioactive compounds indifferent extracts of morinda lucida benth (rubiaceae) root using GC–MS analysis


  • David O. Adekunle Industrial Chemistry Programme, Bowen University, P.M.B. 248, Iwo, Nigeria
  • Esther O. Faboro Industrial Chemistry Programme, Bowen University, P.M.B. 248, Iwo, Nigeria
  • Labunmi Lajide Department of Chemistry, The Federal University of Technology, PMB 704, Akure, Nigeria


GC-MS, M. lucida roots, Bioactive compounds, Anthraquinone


This study aims to analyze and describe the chemical constituents of various crude extracts derived from the root of Morinda lucida Benth (Rubiaceae), an ethnomedicinal plant commonly found in Nigeria. The root of Morinda lucida was dried at room temperature and pulverized into powder. The soxhlet extraction technique was used with solvents of different polarities, namely hexane, chloroform, and methanol, to obtain three distinct extracts. Subsequently, gas chromatography-mass spectrometry analysis was carried out on the extracts. A total of 69 compounds were identified from the different extracts of M. lucida root. The hexane extract had 3 major compounds and 8 minor ones, with diethyl phthalate being the most prominent with 87.10% peak area. The chloroform extract had 24 compounds, with phthalic acid, 2-Ethylhexyl isohexyl ester being the highest with 16.61% peak area. Six of these compounds had more than 5%, while the remaining 18 ranged from 2.50% to 1.00%. The ethanol extract contained 36 compounds, with 6 compounds being greater than 5% and the remaining 30 less than 5%. The highest percentage in the ethanol extract was 2-Pyrrolidinone, 1-methyl-, at 16.05%. In terms of the biological and pharmacology benefits, these chemicals are regarded as crucial. Also, each of the three extracts has a few similarities in their physicochemical properties that can be related to the natural substances that are abundantly found in M. lucida root. The GC-MS analysis of different extracts of M. lucida revealed the presence of several bioactive compounds that have potential therapeutic properties.


F. P. Casuga, A. L. Castillo & M. J. T. Corpuz, “GC–MS analysis of bioactive compounds present in different extracts of an endemic plant Broussonetia luzonica (Blanco) (Moraceae) leaves”, Asian Pac J Trop Biomed 6 (2016) 957.

K. E. Adewole, A. F. Attah & J. O. Adebayo, “Morinda lucida Benth (Rubiaceae): A review of its ethnomedicine, phytochemistry and pharmacology”, Journal of Ethnopharmacology 276 (2021) 114055.

H. O. Lawal, S. O. Etatuvie & A. B. Fawehinmi, “Ethnomedicinal and pharmacological properties of Morinda lucida”, J. Nat. Prod. 5 (2012) 93.

G. Rath, M. Ndonzao & K. Hostettmann, “Antifungal anthraquinones from Morinda lucida”, Int. J. Pharmacogn. 33 (1995) 107.

M. Suzuki, N. H. Tung, K. D. Kwofie, R. Adegle, M. Amoa-Bosompem, M. Sakyiamah, F. Ayertey, K. B. A. Owusu, I. Tuffour, P. Atchoglo, K. K. Frempong, W. K. Anyan, T. Uto, O. Morinaga, T. Yamashita, F. Aboagye, A. A. Appiah, R. Appiah-Opong, A. K. Nyarko, S. Yamaoka, Y. Yamaguchi, D. Edoh, K. Koram, N. Ohta, D. A. Boakye, I. Ayi & Y. Shoyama, ‘New anti-trypanosomal active tetracyclic iridoid isolated from Morinda lucida Benth”, Bioorg. Med. Chem. Lett 25 (2015) 3030.

S. O. Okoha, O. T. Asekuna, O. B. Familonia & A. J. Afolayan, “Composition and Antioxidant Activities of Leaf and Root Volatile Oils of Morinda lucida”, Natural Product Communications 6 (2011).

W. C. Evans & T. Evans, Pharmalognosy (15th edition) W.B Saunders company LTD, London, 2002, pp. 191-393.

J. F. Anderson, F. J. Ferrandino M. P. Vasil, R. H. Bedoukian, M. Maher & K. McKenzie, “Repellency of Naturally Occurring or Related Compounds, DEET, and Para-Menthane-3,8-Diol to Bed Bugs (Hemiptera: Cimicidae)”, J Med Entomol 4 (2018). 55(3):666-672. doi: 10.1093/jme/tjx253. PMID: 29415167.

S. Guang-Yao, C. Ming-Long & W. Kui-Wu “Natural New Bioactive Anthraquinones from Rubiaceae”, Mini-Reviews in Organic Chemistry 17 (2020) 872.

S. C. Chien Y. C. Wu Z. W. Chen & W. C. Yang, “Naturally occurring anthraquinones: chemistry and therapeutic potential in autoimmune diabetes”, Evid Based Complement Alternat Med (2015) 357357.

R. Chen, J. He & X. Tong, “The Hedyotis diffusa Willd. (Rubiaceae): A review on phytochemistry, pharmacology, quality control and pharmacokinetics”, Molecules 21 (2007) 710.

M. Saminathan, R. B. Rai & K. Dhama, “Systematic review on anticancer potential and other health beneficial pharmacological activities of novel medicinal plant Morinda citrifolia (Noni)”, Int. J. Pharmacol 9 (2013) 462.

P. N. Khanh, T. T. Huong & O. Spiga, “In silico screening of anthraquinones from Prismatomeris memecyloides as novel phosphodiesterase type-5 inhibitors (PDE-5Is)”, Rev. Int. Androl. 16 (2018) 147.

S. Jeremic, A. Amic & M. Stanojevic-Pirkovic, “Selected anthraquinones as potential free radical scavengers and P-glycoprotein inhibitors”, Org. Biomol. Chem. 16 (2018) 1890.

F. Isfahani, D. Ajloo & A. Kanaani, “Photoconversion of an anthraquinone derivative in the presence of human serum albumin. Spectrochim”, Acta A Mol. Biomol. Spectrosc. 205 (2018), 298.

M. Balasubramanian & J. G. Keay, “In Comprehensive Heterocyclic Chemistry II; A. R. Katritzky, C. W. Rees & E. F. V. Scriven, (Ed.) Pergamon, New York, 1996, pp. 245- 266.

K. Raut, R. Thombare, P. Zagade & N. Kumbhar, “Different biological activities of quinoline”, World Journal of Pharmaceutical Research 9 (2020) 674.

A. Abdou, A. Elmakssoudi, A. E. Amrani, J. JamalEddine & M. Dakir, “Recent advances in chemical reactivity and biological activities of eugenol derivatives”, Medicinal Chemistry Research 30 (2021) 1011.

D. O. Adekunle, A. O. Olanrewaju & A. I. Odugbemi, “GC and FTIR studies of hexane and ethanol extracts of Calophyllum Inophyllum, Nigeria”, International Journal of Innovative Research and Development 8 (2019) 197.

E. O. Faboro, L. Wei, S. Liang, A. G. McDonald & C. A. Obafemi, “Characterization of dichloromethane and methanol extracts from the leaves of a medicinal plant: Globimetula oreophila, Industrial Crop and Products 83 (2016) 391.

S. Takatsuto, B. Ying, M. Morisaki & N. Ikekawa, “Microanalysis of brassinolide and its analogues by gas chromatography and gas chromatography-mass spectrometry”, Journal of Chromatography A 239 (1982) 233.

C. V. Jayachandran Nair, S. Ahamad, W. F. Khan, V. Anjum & R. Mathur, “Gas chromatography-mass spectrometric determination of components of leaves of Aegle marmelos and Psidium guajava and seeds of Nigella sativa and correlation with In vitro antioxidant activity”, Pharmacognosy Research 10 (2018) 230.

M. I. Razborcek, “Determination of major phenolic acids, phenolic diterpenes and triterpenes in rosemary (Rosmarinus officinalis L.) by gas chromatography and mass spectrometry”, Acta Chimica Slovenica 54 (2007) 60.



How to Cite

Identification and quantification of bioactive compounds indifferent extracts of morinda lucida benth (rubiaceae) root using GC–MS analysis. (2023). Journal of the Nigerian Society of Physical Sciences, 5(4), 1534.



Original Research

How to Cite

Identification and quantification of bioactive compounds indifferent extracts of morinda lucida benth (rubiaceae) root using GC–MS analysis. (2023). Journal of the Nigerian Society of Physical Sciences, 5(4), 1534.