Statistical analysis of nuclear magnetic resonance relaxation parameters of rare deoxyribonucleic acid intermediates

John A. Lazarus; Michael O. Dada; Awojoyogbe O. Bamidele; Simon O. Abolarinwa

doi:10.46481/jnsps.2027.3527

Authors

John A. Lazarus [email protected] Department of Physics, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria; Department of Physics, Ibrahim Badamasi Babangida University, P.M.B. 11, Lapai, Niger State, Nigeria
Michael O. Dada Department of Physics, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria
Awojoyogbe O. Bamidele Department of Physics, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria
Simon O. Abolarinwa Department of Animal Biology, Federal University of Technology, P.M.B. 65, Minna, Niger State, Nigeria

Keywords:

NMR relaxation times, Rare DNA intermediate structures, Wolfram Mathematica software, Statistical analysis, DNA secondary structures

Abstract

Rare DNA intermediate structures are essential for biological processes in living organisms, including protein folding and unfolding, gene transcription, and replication. Studies have been conducted on DNA intermediate structures using different nuclear magnetic resonance (NMR) approaches, but NMR-based statistical analysis of DNA is less researched. Therefore, the current study presents a statistical analysis of NMR longitudinal (T₁) and transverse (T₂) relaxation times for rare DNA intermediate structures. Published experimental T₁ and T₂ datasets for five rare DNA intermediate structures were simulated using an R script to obtain 500 data points for each DNA structure; these were used to perform non-parametric statistical analysis using Wolfram Mathematica software. Results of the analysis show that the T₁ and T₂ datasets for each of the five rare DNA structures have distinct features. This highlights the possibility of utilising DNA T₁ and T₂ NMR parameters to isolate DNA structures responsible for regulating gene transcription, replication, and repair.

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